RNA-seq Analysis of the BCG Vaccine in a Humanized Mouse Model

Objective: This study was aimed at screening differentially expressed genes (DEGs) and exploring the potential immune mechanism induced by Bacillus Calmette-Guerin (BCG) vaccine in a humanized mouse model. Methods: Candidate DEGs between mice vaccinated with BCG or injected PBS were identified through transcriptomics, their biological functions, signaling pathways, protein interaction networks analyzed bioinformatics. Results: A total of 1035 transcriptomics: 398 up-regulated 637 down-regulated. GO analysis indicated that these significantly enriched cell adhesion, oxygen transport, receptor complex, carbohydrate binding, serine-type endopeptidase activity, peroxidase activity terms. KEGG involved Rap1 pathway, axon guidance, PI3K-Akt natural killer mediated cytotoxicity, cytokine-cytokine interaction. Protein network demonstrated Myc, Vegfa, Itgb3 proteins had highest aggregation degree, coefficient, connectivity. Conclusion: The mice. Among them, down-regulated myc itgb3 pathway may play essential roles vaccine.